鼻咽癌病人血清凝集因子检测

The Assay of Agglutination Factor in Sera of Patients withNasopharyngeal Carcinoma(NPC)

作者采用自行设计的方法，首次在鼻咽癌病人血清中检测出凝集因子（ＡＦ）。鼻咽癌病人血清以Ｄ－Ｈａｎｋｓ缓冲液倍比稀释（１：２０～２５６０）。人鼻咽癌细胞株（ＣＮＥ－２）经胰蛋白酶消化，戊二醛固定制成单个细胞悬液。两者混合于９６孔培养板，５０００细胞／孔，经３７℃、４℃、再３７℃处理后，在倒置显微镜下观察，以能形成一个≥２０细胞的凝集团块为ＡＦ阳性，结果发现２３１例鼻咽癌病人血清都是阳性，几何平均滴度（ＧＭＴ）１：２６５．１。１１１名正常人中７４人ＡＦ阴性，３７人低滴度阳性（１：２０～１６０），ＧＭＴＩ：３９．２。提示鼻咽癌病人血清ＡＦ水平明显高于正常人（Ｐ＜０．００１）。作者对结果及方法进行了讨论。

Using a new method established by themselves , the authors detected agglutination factor(AF)in sera of nasopharyngeal carcinoma(NPC)patients.The sera of NPC patients were diluted by multiple propor-tions(1:20～2560)with D-Hanks buffer solution. Single cell suspensions of CNE-2 cells were prepared aftertrypsinization and glutaraldehyde fixation. Both of them were mixed up into 96 welI microculture pIate, 5000cells per well,then the plate was incubated at varying temprature(37℃-4℃-37℃). The cell agglutinationwas observed under inverted microscope,so long as formed one agglutinative cell clump(≥20 cells)in a well,the result was considered as positive The results showed that in 23l cases of NPC AF were positive,geometri-cal mean titre(GMT) was 1:265.1.Among 111 cases of healthy controls,74 were negative, 37 were positivewith low titre(1:20～160), GMT was 1: 39.2.It is Obvious that the AF Ievels in serum of NPC patientswere significant higher than that of the healthy controls(P<0.00.In this paper the AF phenomenon andmethodology were discussed.