着床前后兔子宫内膜糖蛋白变化的电泳分析

Studies on the Character of Glycoproteins of Rabbit Endometrium During Implantation Stage

本文以二种凝集素ＷＧＡ、ＬＣＡ为探针，通过ＳＤＳ－ＰＡＧＥ、印迹转移及ＡＢＣ免疫酶标染色方法，连续观察了着床期间（Ｄ５－Ｄ９，交配日为Ｄ０）兔子宫内膜糖蛋白表达的动态变化。发现ＷＧＡ结合４１Ｋｄ、８５Ｋｄ，ＬＣＡ结合４５Ｋｄ、８３Ｋｄ糖蛋白在胚泡进入子宫腔，但尚游离时（Ｄ５，Ｄ６）开始升高，在着床始发的Ｄ７天达高峰，胚泡植入后（Ｄ７．５，Ｄ８）下降，着床完成时（Ｄ９）多已消失。其中４１ＫｄＷＧＡ结合和４５ＫｄＬＣＡ结合特异性糖蛋白变化尤为突出，其含量在未孕时极微，Ｄ７显著增高，着床开始后（Ｄ７．５）即迅速下降，且这两条带主要存在于胚泡着床侧。结果表明：着床期间子宫内膜表面糖蛋白有阶段特异性变化，并该变化可能受到胚泡的局部调节作用。

BY SDS-PAGE,Western blot,and ABC immune enzyme-labled assay,we studied the dynamic change of glycoprotein expression on rabbit endometrium at peri-implantation stage.Lectins including WGA,LCA,were used as probes,The results showed that 41Kd and 85Kd WGA,45Kd and 83Kd LCA-binding glycoprbteins began to increase from preimplantation stage(D5,D6),and reached their highest levels at implantaton stage(D7),respectively,then decresed D7.5.D8,and disappeared at post-implantation(D9)stage.Among them,it was more noticeable that changes of two glycoproteins binding to WGA(41Kd)and to LCA(45Kd) were more obvious,and primarily seen on anti-mesometrial region.These reaults indicate that stage-specific change of glycoprotein expression occuring on rabbit endometrium, and may be related to the regulation of blastocyst.