用地高辛标记的染色体特异性DNA探针检测石蜡切片组织细胞中期核染色体异常的敏感方法

A Sensitive Method of Using Digoxigtnin-labelled Chromosome-specific Probes to Detect the Interphase Chromosome Aberration on Paraffin-embedded Tissue Sections

为了增强福尔马林固定，石蜡包埋组织切片中细胞中期核染色体原位杂交效果，我们对组织的预处理条件进行了改良，结果发现，应用微波加热，硫氰酸钠处理后蛋白酶消化时间明显缩短，减轻了对组织形态结构的破坏，在大多数切片中获得均匀一致的杂交信号。提示本改良方法可能成为肿瘤发生学研究的重要工具。

In order to improve the efficiency of in situ hybridization(ISH) with digoxi-genin- labelled alpha satellite DNA probes specific for the centromeric reglons of chromo-somes 7 and 17 in routinely processed paraffin-embedded tissue sections we studied the condi-tions of tissue pretreatment related to ISH.It was found that pretreatment with nucrowave heating and sodium thiocyanate prior to pepsin digesting could significantly reduced the dam-age to the histologic morphology and markedly enhanced the reproducibility of ISH reaction.More than 80% of the tumor cells and lymphocytes showed distinct and more homogenous chromosome hybridization signals in the most of 4μm-thick sections,suggesting that the modified protocol could be an itnportant tool for determing chromosome aberrations in paraf-fin-embedded tissue sections.