p53蛋白在喉癌及癌旁病变组织中的表达

p53 Protein Expression in Laryngeal Carcinoma and Its Surrounding Lesions

应用抗突变型ｐ５３单抗对来自２１例喉癌病人的癌及癌旁病变组织进行了免疫组化研究。结果表明：ｐ５３蛋白在癌旁正常粘膜、单纯增生、非典型增生及癌变组织中的阳性率分别是１４．３％、２０．８％、４７．１％和５２．４％，阳性率呈递增的趋势，ｐ５３蛋白在各病变组织中的表达强度也逐渐提高。说明ｐ５３表达的改变可发生在喉粘膜癌变的早期，ｐ５３的过度表达可作为诊断喉癌早期病变的一个生物指标。

In order to define the relationship between p53 protein expression and the malignant transformation of laryngeal epithelium,both carcinomas and the epithelium adja-cent to carcinoma were studied by immunohistochenustry using mutant form p53 monoclonal antibody.3 of 21(14.3%)samples of the normal epithelium adjacent to tumors,5 of 24(20.8%)samples of the hyperplasia,8 of 17(47.1%)samples of the dysplasia,and 11 of 21(52.4%)samples of the carcinoma were shown to express p53.Positive expression rate as well as the intensity of p53 expression increased gradually as tissue abnormalities progressed.We conclude that p53 expression can be altered very early in the pathogenesis of laryngeal carcinoma and the accumulation of p53 protein may be an early biomarker for identifying high-risk subjects of laryngeal cancer.