高效液相色谱法测定玉米样品中的柄曲霉毒素

High Performance Liquid Chromatogrphic Determination of Trace Sterigmatocystin in Corn

本文研究了应用高效液相色谱法测定玉米样品中痕量柄曲霉毒素的新方法。样品先用甲醇：水（１：１）浸取，环已烷除油脂．再用二氯甲烷萃取毒素，然后将二氯甲烷在真空中蒸发至干，用乙腈溶解器内壁残留物并定容供分析用。柄曲霉毒素的乙腈溶液用反相色谱系统分离，乙腈／水（５５／４５，Ｖ／Ｖ）作流动相，紫外检测器检测（λ＝３２５ｎｍ），检出限ｌｐｍｏ１，回收率７６．５％。

A precise and sensitire HPLC method for determination of sterigmatocystin in spiked corn was reported. Sterigmatocystin was extracted from sample with methanol-water and filtered. The filtrate was defatted with cyclohexane and then partitioned in dichloromethane. The extracts were concentrated to dryness and dissolved in acetonitrile, which have been separated on C18 column with acetonitrile / water (55 / 45,V / V) as mobile phase and detected by UV spectrophotometry (λ= 325nm). The detection limit and recorery of sterigmatocystin in spiked corn sample are 1 pmol / L and 76.5%respectirely.