用微弹轰击法介导的转基因对小鼠进行乙肝病毒遗传免疫研究

Study on Genetic Immunization of Hepatitis B Virus in Mice by Particle Bombardment-mediated Gene Transfer

用包裹ＤＮＡ的金微弹轰击耳朵，将表达乙型肝炎病毒表面抗原（ＨＢｓＡｇ）基因的重组质粒转入小鼠皮肤细胞，在小鼠血清中检测ＨＢｓＡｇ及抗－ＨＢｓ抗体。实验组８只小鼠中，有３只在基因转移３天后检测到ＨＢｓＡｇ。１４天再轰击１次。１６天后２只鼠中检测到抗－ＨＢｓ抗体。２８天后４只鼠中检测出抗ＨＢｓ抗体。对照组４只小鼠中ＨＢｓＡｇ及抗－ＨＢｓ抗体均未检测出。本实验为今后进一步分析乙肝病毒的遗传免疫奠定了实验基础，提供了实验依据。

A plasmid construct expressing hepatitis B virus surface antigen (HBsAg) was introduced into the skin cells of living mice by bombarding the ears with DNA-coated gold particles. HBsAg and anti-HBs antibody were assayed in sera of the mice following gene transfer.Three days after introduction of the plasmid,HBsAg appeared in 3 of the 8 treated mice. On day 14,the mice were rebombarded again and anti-HBs antibody was detected in two of the 8 treated mice 2 days after the second bombardment. On day 28, anti-HBs antibody was found in 4 of the 8 immunized mice. Neither HBsAg nor anti-HBs antibody was observed in the 4 controls.