前列腺特异抗原免疫放射分析反应的动力学研究

Study on Kinetics of Immunoradiometric Assay for Prostate Specific Antigen

本文着重对前列腺特异抗原（ＰＳＡ）的免疫放射分析的反应时间进行研究。实验采用两步，一为固定第一步时间，改变第二步反应时间，另一为固定第二步时间，改变第一步反应时间，观察反应的变化。结果表明，第一步反应０．５小时以后，标准和质控的ｃｐｍ已相对恒定，０．５小时的最大结合率为５８．７％，与６小时的最大结合率５５．８％相比，差异不显著。第二步反应，从１小时到６小时，结合率随时间的增加而升高。１小时的结合率为４６．３％，与６小时的结合率７２．２％相比，差异显著。此结果将有助于对ＰＳＡ的临床检测提供一个最适宜的反应时间。

Kinetics of PSA IRMA was studied by two ways: fixing the interval of first incubation with varying second incubation period and fixing the interval of second incubation with varying first ones. The maximum binding level kept constant after a period of half an hour for first incubation and there was no difference between B/T at 1/2h (58.7%) and that at 6h (55.8%). However,a steady increase in B/T from 43. 3% at lh to 72.2% at 6h was noted with lengthening the interval for second incubation. It is concluded that a period of 2h at first incubation and 6h at second incubation is optimal for the best performance of PSA IRMA developed in our laboratory.