摘要
以抗卵巢癌肿瘤抗原的单克隆抗体ＯＶ１９７作为捕捉抗体，ＯＶ１８５作为标记抗体，建立了特异性和灵敏度高的碘标固相双位点夹心式的ＣＡ－１２５免疫放射分析（ＩＲＭＡ）。本文报导了ＣＡ－１２５ＩＲＭＡ的动力学研究。ＣＡ－１２５抗原与捕捉抗体ＯＶ１９７室温３～４小时达到平衡；而固相ＯＶ１９７抗体结合的的ＣＡ－１２５抗原与标记抗体ＯＶ１８５室温孵育２～３小时达到平衡。反应时振荡与否、振荡频率大小以及捕捉抗体和标记抗体的量等都对ＩＲＭＡ有一定影响。这就为ＩＲＭＡ的理论研究提供了基础，并对ＩＲＭＡ的实际操作起到了指导作用。
Kenetics of IRMA for CA-125 using anti-CA-125 McAb OV197 as capture antibody and OV185 as labeling antibody was investigated, which showed that reaction equilibrium of OV197 and CA-125 was reached within 3～4 hours and OV185 within 2～3 hours. Agitation frequency and the amount of tracer and capture antibody also had influence on the kenetics of CA-125 IRMA.
出处
《标记免疫分析与临床》
CAS
1995年第3期158-160,共3页
Labeled Immunoassays and Clinical Medicine
关键词
免疫放射分析
抗原
动力学
卵巢肿瘤
诊断
Immunoradiometric assay
Antigen,tumor-associated
carbohydrate
Ovarian neoplasms
Kenetics/immunology
