摘要
本文所介绍的T4-BA-ELISA及T3-BA-ELISA分析,其灵敏度已达到甚至超过了放射免疫分析的灵敏度,标准曲线范围与放射免疫分析相同。标准曲线的线性良好,血清稀释曲线、回收率及批内、批间变异系数均符合免疫分析的质量控制要求。操作简便、快速,保存期长,避免RIA使用放射性同位素及保存期短的缺点。分别检测123例及114例正常人血清的总T4及总T3值,与有关文献报导的T4-RIA、T3-RIA的结果基本相符。用本方法测定甲亢及甲低病人血清总T4及总T3浓度,升高和降低与临床诊断相符合。
BA-ELISA for serum total T4 and T3 presented in the paper had a sensitivity similar or even superior to the conventional RIA and a measurable range same as that of RIA. The standard curve,accuracy and precision were found to conform to the demands of quality control through the evaluation of recovery rate,serum dilution curve and coefficients of variation. The mean of normal serum level of total T4 and T3 was 109.4±25.7nmol/L (8.5±2.0μg/dl, n=123),and 2.12±0.46nmol/L (138±30ng/dl, n=114), respectively.Serum concentration of T4 and T3 in hyperthyroidism and hypothyroidism measured by the assay was consistent with the clinical diagnosis.
出处
《标记免疫分析与临床》
CAS
1995年第4期210-213,共4页
Labeled Immunoassays and Clinical Medicine
关键词
甲状腺疾病
T3
T4
BA-ELISA
Triiodothyronine
Thyroxine
Enzyme-linked immunosorbent assay
Biotin
Avidin