人血清总甲状腺素（T_4）和总三碘甲腺原氨酸（T_3）生物素亲合素定量酶联免疫分析（BA－ELISA）

Biotin-avidin Enzyme-linked Immunosorbent Assay (BA-ELISA)for Serum Total Thyroxine (T_4) and Triiodothyronine (T_3)

本文所介绍的Ｔ４－ＢＡ－ＥＬＩＳＡ及Ｔ３－ＢＡ－ＥＬＩＳＡ分析，其灵敏度已达到甚至超过了放射免疫分析的灵敏度，标准曲线范围与放射免疫分析相同。标准曲线的线性良好，血清稀释曲线、回收率及批内、批间变异系数均符合免疫分析的质量控制要求。操作简便、快速，保存期长，避免ＲＩＡ使用放射性同位素及保存期短的缺点。分别检测１２３例及１１４例正常人血清的总Ｔ４及总Ｔ３值，与有关文献报导的Ｔ４－ＲＩＡ、Ｔ３－ＲＩＡ的结果基本相符。用本方法测定甲亢及甲低病人血清总Ｔ４及总Ｔ３浓度，升高和降低与临床诊断相符合。

BA-ELISA for serum total T4 and T3 presented in the paper had a sensitivity similar or even superior to the conventional RIA and a measurable range same as that of RIA. The standard curve,accuracy and precision were found to conform to the demands of quality control through the evaluation of recovery rate,serum dilution curve and coefficients of variation. The mean of normal serum level of total T4 and T3 was 109.4±25.7nmol/L (8.5±2.0μg/dl, n=123),and 2.12±0.46nmol/L (138±30ng/dl, n=114), respectively.Serum concentration of T4 and T3 in hyperthyroidism and hypothyroidism measured by the assay was consistent with the clinical diagnosis.