雌激素及XW_（630）对类成骨细胞增殖及碱性磷酸酶活性的影响

The influence of 1 7β-estradiol and XW630 on proliferation and alkaline phosphatase acivity in osteoblast

雌激素可有效预防妇女绝经后骨质疏松，关于雌激素对骨形成过程的影响尚存争议。我们应用成骨细胞样骨肉瘤细胞株ＵＭＲ－１０６细胞在细胞水平探讨１７β雌二醇（Ｅ２）对骨代谢的调节作用，同时观察新型抗骨质疏松药物ＸＷ６３０对成骨细胞的影响。Ｅ２１０９～１０７ｍｏｌ／Ｌ显著刺激ＵＭＲ－１０６细胞３Ｈ－ＴｄＲ掺入及细胞计数，并使细胞浆及分泌的碱性磷酸酶（ＡＫＰ）活性显著提高。Ｅ２１０－１０ｍｏｌ／Ｌ对ＵＭＲ－１０６细胞增殖及活性无影响。与Ｅ２比较，ＸＷ６３０１Ｏ－１０～１０－７ｍｏｌ／Ｌ均可显著刺激ＵＭＲ－１０６细胞的增殖及活性，且作用强于Ｅ２。结果表明，Ｅ２可刺激成骨细胞增殖及活性，百ＸＷ６３０可能成为治疗骨质疏松的有效药物。

bjective :To investigate the effect of 17β-estradiol(E2)and XW630 on proliferation and AKP activity of the osteoblast- like osteosarcoma cell line UMR 106 cells.Design :In vitro experimental study. Setting :Laboratory in Department of Pharmacology, Peking Union Medical College and Chinese Academy of Medical Scien Methods: UMR-106 cells were cultured in mixture of DMEM/F-12 medium with FCS ,and passaged with trypsin and EDTA,3H- thymidine was incorporated and radioactivity was counted.AKP activity was assayed in Technicon RA 2000 instrument.Results :E2 significantly increased3H- thymidine incorporation ,cell number ,intracellular and excreted AKPactivity.At the same time,we compared the effect of a new drug XW630 with that of E2.XW630 ex- hibited more potent effects.Conclusions :The results suggested that E2 elevated proliferation and activity of UMR-106 cells and XW6 30 may become a new way treating postmenopausal osteoporosis.