丙型肝炎病毒──石蜡包埋肝组织原位杂交

DETECTION OF HEPATITIS C VIRUS BY IN SITU HYBRIDIZTION

为探讨ＨＣＶ感染的组织病毒学表现，以狄高辛素标记ＨＣＶｃＤＮＡＣ亚基因探针，对３４例血清学诊断的非甲非乙肝炎病人的石蜡包埋肝组织进行原位杂交。检出乙型肝炎４例，丙型肝炎１４例，乙型和丙型混合１３例。乙型肝炎均ＨＢｓＡｇ（—）。ＨＣＶ（＋）细胞多肿胀变性，常有淋巴细胞包绕，其分布邻近汇管区或炎症病灶。提示ＨＣＶ感染同时致细胞病变和免疫介递损害。

To examine the histo-virology of HCV infection l the virus RNA was tested in paraffin-embeddedbiopsied liver from 34 patients with non-A, non-B hepatitis by in situ hybridization using digoxinin la-belled cloned cDNA/C gene probe. Of them 4 were hepatitis B,14 hepatitis C,and l3 coinfected withboth HBV and HCV,The HCV-positive hepatocytes were swelling,degenerative, often surroundedwith lymphocytes, and distributed closely to portal region and inflammatory lesion,suggesting that HCVinfections were cytopathogenic and resulted in immunopathogenesis。