实验性小胆管样上皮细胞增殖及其演变的超微结构研究

UITRASTRUCTURAL STUDY ON PROLIFERATION AND MODIFICATION OF EXPERI-MENTAL BILE DUCTULOID EPITHELIAL CELLS

大鼠胆总管结扎术后１至３５天，按不同时间取肝组织，在光学显微镜及电镜下观察其病理和超微病理变化。发现术后第２天在汇管区就可见到小胆管样上皮细胞出现，随着时间的推移不断增殖，从小叶周边延伸进入小叶内部。伴随这类细胞增殖。其周围胶原纤维增生，而且小胆管样上皮细胞本身出现分化演变。其中多数分化形成小胆管，并且多级分枝。这种小胆管和相伴随的胶原纤维一同分割改建肝小叶形成假小叶。少数这类细胞分化演变成管泡状结构的小肝细胞或肝细胞。研究结果支持作者关于小胆管样上皮细胞是增殖了的肝脏干细胞以及它们的增殖演变参与了肝硬化形成和发展的见解。

he Ultrapathological changes of liver were observed in rats whose common bile duct had irrevers-ibly ligated for 1-35 days by electron microscopy.It was found that bile ductuloid epithelial cells were occurred in potal areas at the second day after ligation. The cells proliferated continuously and ex-tended from perilobule into intralobule of time. The collagenous material were proliferated around the hyperplastic ductoid lining cells.The bile ductuloid cells were under differentiation and modification. The majority were modified to bile ductules,its branches and accompanying collagen fiber carved up and reconstructed the lobules into pseudulobules,and a small number of them became small hepatocytes or hepatocytes surronding the lumen of ductlike or alveoluslike structure. This result further supported the veiwpoint of the auther that bile ductuloid epithelial cells were the proliferating liver stem cells and their proliferation and modification participated in development of liver cirrhosis.