摘要
采用逆转录巢式PCR技术检测了51例HCC患者的肝癌及癌周肝组织中HCVRNA正负链,同时以巢式PCR技术检测了这些组织中的HBVDNA。结果,11.8%(6/51)检测出组织中HCVRNA正链;其中5例在癌周肝组织,2例在癌组织中检测出HCVRNA负链。由于负链RNA为HCV的复制中间体,不释放到细胞外,其检出为HCV感染与HCC关系的研究提供了进一步的病因证据。组织中HBVDNA检出率高达92.2%(47/51),显示华南地区HBV感染在HCC的发生中起着特别重要的作用。
Using reverse
transcription(RT)nested polymerase chain reaction(PCR)technl que,the
cancerous and pericancerous tissues from 51 patients with hep
atocelluiar carcinoma(HCC)have been detected for plus and minus
strands of HCV RNA and HBV DNA,6 patients specimens were positive of
plus strands of HCV RNA, in which the minus strands of HCV RNA were
found in 5 pericancerous specimens of liver tiSsue and 2
cancerous specimens.B ecause the minus stranded RNA is the
replicative intermediate of HCV and can not be released to the
outside of hepatocytes,its detectlon would Indicate the presence and
replication of HCV inside hepatocytes.These results also demonstrated
the relationship between HCV andHCC. The positlve rate of HBV DNA in
HCC tissues detected by nested PCR was as hlgh as 92.2%,which
indicated the iniportance of HBV in the development of HCC in
Southern China.
出处
《肝脏病杂志》
CSCD
1995年第4期202-204,共3页
基金
国家教委博士点基金
关键词
肝肿瘤
HCV
HBV
聚合酶链反应
病毒基因
Liver neoplasms Hepatitis C virus Hepatitis B
virus Polymerase chain reaction