摘要
应用ＨＥＶ基因结构区的重组融合蛋白，建立了免疫吸印法（ＷｅｓｔｅｒｎＢｌｏｔ．ＷＢ）检测抗－ＨＥＶＩｇＧ，并与现行的酶联免疫试验（ＥＩＡ）进行了比较。结果表明，ＷＢ检出戊型肝炎暴发点急性肝炎病人血清抗－ＨＥＶＩｇＧ的阳性率（６８／７３，９３．２％）高于现行的ＥＩＡ（５１／７３，７００％），对实验感染猕猴抗－ＨＥＶＩｇＧ检出率前者也高于后者（分别为７／７和５／７），且检出抗－ＨＥＶＩｇＧ时间较长，滴度也较高。对多种对照血清检测结果表明，除５０％（３／５８）的丙型肝炎血清呈阳性外，甲型、乙型、ＥＢＶ、ＣＷＶ肝炎及健康人血清均为阴性，说明该法具有良好的灵敏度和特异性，可望用于流行病学调查和基础研究。
Western blot assay(WB) was developed for detection of anti-HEV IgG
using a recombinant fu-sion protein encoded by thc 3-terminus of ORF2
of hepatitis E virus(HEV)genome,68 of 73 acute hepatitis E(HE)sera
were anti-HEV IgG positive by WB,with an overall positive rate of 93.
2%,com-pared with 70%(51/73) by EIA。In l0 hepatitis A,18 hepatitis
B,10 CMV Infection,10 EBV infections and 18 healthy controls nobodies
were Ieactive,whilc 3 of 58 hepatitis C were positive by this assay.
Serial sera from 7 experimental infected Rhesus monkeys were also
tested for ant-HEV IgG by WB.All of 7 monkcys wcre sero conVerted
immediately after ALT elevation by EIA。The results indicate that
this assay appears more sensitive and speclflc for HE and may be used
for thc epidemiological and basic studies on HEV infection,also can
be as a confirmation test for HE diagnosis.
出处
《肝脏病杂志》
CSCD
1995年第4期216-218,共3页
