肿瘤坏死因子在肝损害中的作用

ACTION OF TUMOR NECROSIS FACTOR IN LIVER INJURY

我们利用动物体内外试验，研究了肿瘤坏死因子α（ＴＮＦα）在肝损害中的作用。结果显示，ＴＮＦα单独或与Ｄ－氨基半乳糖（Ｄ－ＧａｌＮ）联合作用于培养肝细胞，在一定剂量范围内，上清中ＡＳＴ活性无明显变化；将ＴＮＦα处理后树血清作用于培养肝细胞，细胞上清中ＡＳＴ活性升高，加入一定浓度的抑肽酶能抑制这种效应。在ＨＢｓＡｇ预先刺激的树体内注入ＴＮＦα，可导致肝、肺、肠等器官的出血、坏死。这提示，ＴＮＦα在肝损害中起一定作用，并在体内外存在差异。

n this paper the action of TNF- αin llver injury was studied In vivo and in vitro,The results revealed that TNF-α alone or with D-GaIN in cultured hepatocytes couldn’t change the AST activity in the supernatant under certain dosages,but the sera of TNF-treated Tupaia could raise AST activity in cultured hcpatocyte supernatant, and the increased AST activity could be depressed by Aprotinin.If TNF-α was inlected into the animals treated wIth HBsAg through portal vein,which could induce hemorrhage and necrosis in some organs such as liver,lungs or intestine, etc。 These findings suggested that TNF-α can induce liver damage,and its action is difference between vivo and vitro