神经生长因子对豚鼠庆大霉素耳毒性的保护作用

The protective effect of nerve growth factor against ototoxicity of gentamycin in guinea pig

本研究应用光镜及电镜观察了神经生长因子（ＮｅｒｖｅＧｒｏｗｔｈＦａｃｔｏｒ，ＮＧＦ）对豚鼠庆大霉素耳毒性的保护作用。结果表明，Ａ组动物在肌注庆大霉素（１５０ｍｇ／Ｋｇ）１４天后，Ｃｏｒｔｉ＇ｓ器的外毛细胞大片坏死，琥珀酸脱氢酶阳性细胞数明显减少，残存的毛细胞及神经纤维严重变性；Ｂ组动物在肌注同等剂量庆大霉素的同时给予ＮＧＦ（２０００ＢＵ／Ｋｇ）１４天后，Ｃｏｒｔｉ＇ｓ器的毛细胞及神经纤维的损伤明显轻于Ａ组。测定动物给药前后的听阈，Ｂ组的听力损失亦明显轻于Ａ组。由此证明ＮＧＦ对庆大霉素所致的内耳损伤具有明显的保护作用。

By using histochemical and electric microscopic methods the protective effect of nerve growth factor(NGF) against ototoxicity of gentamycin was studied.The animals were divided into two groups.In the group A the animals were injected with gentamycin(150mg/Kg) for 14 days.In the group B the animals were injected with gentamycin (150mg/Kg) and NGF (2000BU/Kg) for the same time.After administration of above drugs the funetional and morphologic changes were observed.The results showed that in the group B the degrees of hearing loss and lesion of hair cells and nerve fibers of Corti's organ were slighter than those in the group A.It is indicated that NGF can weaken ototoxicity of gentamycin.