原位免疫PCR技术及对胃癌神经内分泌分化的研究

Immuno-in situ PCR and detection of neuroendocrine differentiation of gastric carcinoma

原位免疫ＰＣＲ技术是将合成生物素标记的外源性ＤＮＡ片段通过卵白素与生物素标记抗体结合，进行原位ＰＣＲ反应，使外源性ＤＮＡ片段作为放大信号，再应用免疫组化ＡＢＣ法显示信号。本文应用原位免疫ＰＣＲ技术及免疫组化方法对２２例胃癌的嗜铬粒蛋白Ａ（ＣｇＡ）进行观察，结果发现免疫组化ＣｇＡ阳性率为３８．６％，原位免疫ＰＣＲ为８５．７％，证明胃癌中含有神经内分泌细胞是很常见的现象。本文对原位免疫ＰＣＲ技术进行了介绍和讨论。

Immuno-in situ PCR is a technique in which via ovalbumin,a synthetic biotin-labeled exogenous DNA sequence is made to conjugate with a biotin-labeled second antibody to carry out in situ PCR reaction to amplify the exogenous DNA sequence as a signal,which is then color-developed by immunohistochemical ABC method.We used the anti-chromogranin A (CgA) antibody to detect the expression of neuroendocrine differentiation of gastric carcinomas in 22 cases.It is found that the rate of CgA positivity was 38.6% in immunohistochemistry and the rate was 85.7% in immuno-in situ PCR,demonstrating that gastric carcinoma containing neuroendocrine cells is a commonly seen phenomenon.The immuno-in situ PCR technique was introduced and described.