胎盘型谷胱甘肽S－转移酶在人乳腺癌的免疫组化检测

Immunohistochemical examination of placental form of glutathione S-transferase in human breast cancer

应用抗人胎盘型谷胱甘肽Ｓ－转移酶（ＧＳＴ－π）单克隆抗体，以微波ＬＳＡＢ免疫组化技术，检测了ＧＳＴ－π在人乳腺癌中的表达情况。１３２例乳腺癌中，７８．，８％（１０４／１３２）呈阳性表达。ＧＳＴ－π表达与患者年龄、肿瘤大小及腋窝淋巴结转移无明显相关，与组织学类型有一定关系，ＧＳＴ－π在ＥＲ（一）乳腺癌的阳性表达率９１．３％，显著高于ＥＲ（＋）者６４．４％（Ｐ＜０．０１），同样ＧＳＴ－π在ＰＲ（一）乳腺癌的阳性率８８．２％，也显著高于ＰＲ（＋）者６６．７％（Ｐ＜０．０５）。结果表明，ＧＳＴ－π的表达与乳腺癌发生、发展的关系密切，且与ＥＲ和ＰＲ的表达呈明显负相关，文中就其临床意义进行了讨论。

The expression of human placental form of glutathione S - transferase (GST - π) was examined in human primary breast cancer by LSAB - microwave immunohistochemical method using monoclonal antibody raised against GST - π . Of 132 patients with breast cancer, 78. 8% were positive for GST - π staining. GST - π expression was not obviously related to the patients' ages, tumor sizes and axillary nodal metastases, but associated in a certain degree with the histological types. The rate of positive GST - π expression in estrogen receptor - negative [ER(-)] tumors was 91. 3% significantly higher than that 64. 4% in ER(+) tumors (P<0. 01). Similarly, the rate of positive GST - π expression in progesterone receptor-negative [PR (-)] tumors was 88. 2% higher than that 66. 7% in PR(+) tumors (P<0. 05). The results showed that GST - π expression is closely related to the incidence and development of human breast cancer and is negatively correlated to the expressions of ER and PR in breast cancer. The clinical significance of GST - π expression was discussed.