豚鼠膜迷路蛋白抗原性分析

Analysis of Antigenity of Membranous Labyrinth Proteins of Guinea Pigs

应用电泳吸附斑点免疫检测法和改良免疫转印法，发现粗制的豚鼠膜迷路蛋白是一种极为复杂的抗原，十二烷基磺酸钠。聚丙烯酰胺凝胶电泳至少可将其分出２２种成分。６８ｋＤ含有４种不同等电点的成分。Ｗｅｓｔｅｒｎｂｌｏｔｔｉｎｇ法分析可以检出ｌｌ条阳性反应带，包括６８ｋＤ、６０ｋＤ和３２ｋＤ，条带之间存在交叉抗原性，膜迷路蛋白和听神经、耳蜗核及肾脏的蛋白成分之间有交叉抗原性，主要位于９８ｋＤ、６８ｋＤ及２７．４ｋＤ，免疫组织化学研究发现抗膜迷路蛋白抗血清可以和内耳广泛的组织结构发生反应，主要部位是：血管纹的细胞膜和细胞间质及螺旋凸，Ｃｏｒｔｉ器的内外毛细胞及支持细胞的胞膜和胞浆，盖膜以及螺旋神经节的细胞膜。还初步发现抗膜迷路蛋白抗血清可以和克雷白杆菌膜蛋白发生交叉反应。

An electrophoretic dot immunobinding assay and a modified western blotting method were established.The components and antigenity of the membranous labyrinth proteins of guinea pigs were analysed. There are the same epitopes between some components of the antigen,between the anti-gen and extractives of acoustic nerve, cochlear nuclei,kidney and Klebsiella pneumoniae,The antigen distributes in stria vascularis,spiral ligament, spiral ganglion,the organ of Corti and Reissner’s mem-brane etc.