甘薯(Ipomoea batatas (L.) Lam.)及其近缘野生种原生质体的植株再生

Plant Regeneration From Petiole Protoplasts of Sweet Potato (Ipomoea batatas (L.) Lam.) and Its Related Species

对甘薯品种高系14号及其近缘野生种I.triloba L、和I.lacunosa L,进行原生质体植株再生研究。从离体培养植株的叶柄分离出原生质体,将其培养在含有0.05mg/L 2,4-D和0.5mg/L激动素(KT)的MS培养基中,从原生质体获得了高频率的愈伤组织。培养8-12周后,将直径达2—3mm的小愈伤组织转移到添加0.05mg/L 2,4-D的MS培养基上。转移3-6周后,将愈伤组织进一步转移到添加吲哚乙酸(IAA)和6-苄基嘌呤(BAP)的MS培养基上,一些愈伤组织再生出植株。未再生植株的愈伤组织进一步在MS基本培养基上培养,它们也再生出植株。本研究从I.triloba原生质体获得高频率的植株再生;首次从I.lacunosa原生质体再生出植株;从高系14号原生质体也再生出完整植株。

Plant regeneration from protoplasts of sweet potato (Ipomoea batatas (L.) Lam.) cv. Kokei 14 and its related species, I.triloba L. and I.lacunosa L., was studied. Protoplasts isolated from petioles of in vitro grown plants were cultured in a modified MS medium containing 0.05 mg/ L 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.5 mg/ L kinetin (KT). High frequency callus formation from protoplasts was achieved. Eight to 12 weeks after plating, protoplast-derived calli up to 2-3 mm in diameter were transferred onto MS medium supplemented with 0.05 mg / L 2,4-D and cultured for 3 to 6 weeks. When the obtained calli were further transferred onto MS medium supplemented with 3-indoleacetic acid (IAA) and 6-benzylaminopurine (BAP), some of them regenerated plants. The remaining non-shoot forming calli were further cultured on MS basal medium and regenerated plants. In this study, I.triloba protoplasts gave a very high regeneration frequency. Plant regeneration from I.lacunosa protoplasts was reported for the first time. Whole plants were also regenerated directly from Kokei No.14 protoplasts.