摘要
在不渗透阻挡滤纸上,建立了Luminol-H2O2-HRP固体表面化学发光分析法.游离HRP测定的线性范围为6~8000pg,检测限为0.66pg/斑点,测定的相对标准偏差为7.4%(8pgHRP,n=11).对标记的HRP测定结果与ELISA法相一致.
A solid surface chemiluminescence method using the anti-permeate barrier as a solid substrate for the determination of HRP (Horseradish peroxidase) based on Luminol -H2O2-HRP system is described. The linear range for free HRP is 6~8000 pg. The detection limit is 0. 66 pg/spot, and the relatve standard deviation for 8 pg HRP is 7. 4% (n=11). This method has been used for assaying three labeled-HRP complexs and the results is comparable with that of ELISA.
出处
《陕西师大学报(自然科学版)》
CSCD
1995年第2期59-61,共3页
Journal of Shaanxi Normal University(Natural Science Edition)
基金
国家自然科学基金
关键词
固体表面发光
化学发光
辣根过氧化物酶
糖蛋白
solid surface luminescence
chemiluminescence
horseradish peroxidase
microtechnique