用荧光法测定蔬菜中的微量抗坏血酸

Determination of ascobric acid in Vegtables by fluorescence method

对抗坏血酸增强钙黄绿素－ＣＵ（Ⅱ）－ＮＨ４ＳＣＮ体系荧光强度进行了研究．结果表明：在实验条件下，抗坏血酸的浓度在１．０×１０－６～８．０×１０－６ｍｏｌ／Ｌ范围内与体系的荧光强度有良好的线性关系，检测限为１．０×１０－６ｍｏｌ／Ｌ．方法已用于测定蔬菜样品中的微量抗坏血酸．

The fluorescent intensity of system of calcein-Cu(Ⅱ)-NH4SCN can be increased by addition of ascobric acid. The result shows it can be used for the determination of ascobric acid in vegetables. The linear range is 1. 0×10-6～8. 0×10-6 mol/L ascobric acid, the detection limit is 1. 0×10-6 mol/L ascobric acid.