慢性乙肝病毒感染者外周血单个核细胞染色体遭受病毒损害的见证

The Evidence of Viral-Damage of Chromosomes in Peripheral Blood Mononuclear Cells (PBMCs) of People with Chronic HBV-infection

用Ｓｏｕｔｈｅｍ ｂｌｏｔ分子杂交技术，检测９９例ＨＢＶＭ阳性的慢性ＨＢＶ感染者外周血单个核细胞（ＰＢＭＣｓ）内ＨＢＶＤＮＡ的存在状态，其中７１例同时用ＢｒｄＵ标记法检测细胞中姊妹染色体交换（ＳＣＥ）率，观察ＨＢＶ对宿主单个核细胞染色体的损害情况。结果发现４２例被检者（４２．４２％）的ＰＢＭＣｓ中存在ＨＢＶＤＮＡ（游离型或整合型，有的还见到游离的复制中间体），正常对照组则无１例检出ＨＢＶＤＮＡ。被检者ＰＢＭＣｓ的平均ＳＣＥ率明显高于对照组，其中ＨＢＶＤＮＡ（＋）组又显著高于ＨＢＶＤＮＡ（－）组。证明ＨＢＶ的入侵与ＳＣＥ率升高之间有密切关系，提示ＨＢＶ对单个核细胞染色体形成了损害。这一现象可能是慢性乙肝病毒感染者免疫功能紊乱和ＨＢＶ长期不能清除等系列表现的原因之一。

In order to find out whether HBV invasion makes damage to chromosomes in immunocompetent cells or not, a series of examinations were carried out. Peripheral blood mononuclear cells(PBMCs) were isolated from blood samples of 99 chronic HBV infected adults for detecting HBVDNA by means of Southem blot hybridization. Among the above people,71 case were detected for sister chromatid exchange (SCE) frequencies in PBMCs by using BrdU-labeling technique. At the same time, 40 normal adults were detected as controls. The results showed that HBVDNA (free form and integrated form) was detected in cells of 42 people with chronic HBV infection but none in the controls. The positive rate in infected people was 42.42%. Of the 71 cases, the mean SCE scores were 6.867± 1.10 in ASC, 7.785± 1.69 in CH,8.27± 1.54 in HCL and 8.45±1.32 in HCC. Their SCE frequencies were significantly higher than that of the controls (5.37±0.81, F=23.32, P<0.01). The patients' mean SCE scores were significantly higher than that of ASC (P<0.ol). Among 71 cases, mean scores of 33 cases with HBVDNA-positive were higher than that of 38 cases with HBVDNA-negative. It was suggested that there were close relation between HBV invasion and increasing of SCE frequencies (i.e HBVDNA replicating in cell and integrating with cell DNA results in chromosome-damage). It would be of great importance for explaining why there are disordered immunity in HB patients, prolonged viremia, reinfections in donor's liver after transplantation etc.