骨巨细胞瘤中p53基因的异常

p53 GENE STUDY IN GIANT CELL TUMOR OF BONE

应用免疫组化及分子杂交方法研究了１０例骨巨细胞瘤及４例胎儿骨标本。大部分多核巨细胞（ＭＧＣ）及约２０％～４０％的单核基质细胞（ＳＴＣ）对抗巨噬细胞单抗标记呈阳性，胎儿骨中破骨细胞对抗巨噬细胞单抗标记亦呈阳性。在抗ｐ５３蛋白单抗染色中，３０％～６０％的ＳＴＣ标记阳性。ＭＧＣ呈两种标记反应，体积大、核多的ＭＧＣ呈阴性，体积小、核少的ＭＧＣ呈阳性。ＤＮＡ分子杂交发现有１例ＧＣＴ存在部分基因片断缺失。

en giant cell tumor of bone(GCT) and four fetal bone specimens were studied with im-munohistochemical and DNA hybrid methods.The results showed most of the nultinuclear giant cell(MGC),about 20%～40%mononuclear stromal cell(STC)and the osteoclast in fetal bone showed strong positive reaction to anti-macrophage MoAb staining.There were two types of MGC manifesting different reactivity to anti-p53 protein MoAb staining.The dimension of the negative staining MGC was much larger and the number of nuclei there in was several times more than that of the positive ones.Also there were about 30%～60%of STC that were positive to an-ti-p53 protein MoAb lebal.It was supposed that the positive reaction MGC and STC to anti-p53 protein MoAb lebal were truly tumor elements of GCT.In southern blot analysis,part deletion of p53 gene was detected only in one specimen.