白细胞介素6基因转染的白血病细胞体内抗白血病浸润的病理形态研究

PATHOLOGICAL STUDIES ON THE ANTI-INVASIVE CHARACTER OF IL-6 GENE TRANSFECTED LEUKEMIA CELLS

应用白细胞介素６（Ｉｎｔｅｒｌｅｕｋｉｎ６，ＩＬ－６）基因转染的高分泌ＩＬ－６的ＦＢＬ－３红白血病克隆株体外扩增后，接种至Ｃ５７ＢＬ／６小鼠，通过动态常规病理检查，观察了ＩＬ－６基因转染的高分泌ＩＬ－６的ＦＢＬ－３细胞（ＦＢＬ－３－ＩＬ－６＋）体内抗白血病细胞浸润的作用。结果表明，接种野生型ＦＢＬ－３细胞的对照组小鼠第７天即在局部出现肿瘤，第１４无可见明显的横纹肌浸润，第２１天出现骨骼及骨髓浸润，第２８天出现肝脏和脾脏浸润，４０天生存数为０。而接种ＦＢＬ－３－ＩＬ－６＋细胞的小鼠的横纹肌、骨骼、骨髓、肝脏和脾脏浸润分别较对照组晚出现７天，４０天生存数为６／８，其中１只存活６０天且未发生骨骼、骨髓、肝脏和脾脏浸润。研究证实，ＦＢＬ－３－ＩＬ－６＋细胞体内接种后能显著延缓和对抗白血病细胞浸润，为该细胞用作瘤苗治疗白血病提供了实验依据。有关ＦＢＬ－３－ＩＬ－６＋细胞抗白血病细胞浸润的机理需进一步研究。

AbstractFBL-3 Leukemia cells transfected with IL-6 gene were expanded in vitro and inoculated into C57BL/6 mice subcutaneously. Tumor growth was observed and histologic analyses of the tu-mors in situ and the liver,spleen and bone marrow were performed at 14, 21, 28 and 35 days af-ter inoculation. The mice inoculated with wild-type FBL- 3 leukemia cells were used as the con-trol.We found that the tumor invasiveness in the mice inoculated with FBL-3-IL-6+ occurred la- ter than in the control group.the survival time of experimental mice was longer than in the con-trol mice. The results demonstrated that inoculation of IL-6 high-secreting FBL-3 inhibited inva-siveness of the leukemia cells,suggesting that the IL-6 gene transfected FBL-3 cells can be used as a vaccine to treat leukemia. The mechanism of the anti-invasiveness of IL-6 gene transfected leukemia cells needs further study.