中国汉族正常人群HLA－B_（27）亚型的分布调查

AN EPIDEMIOLOGICAL STUDY ON HLA-B_(27) SUBTYPING IN HEALTHY CHINESE HAN NATIONALITY

利用聚合酶链式反应（ＰＣＲ）和寡核苷酸探针斑点杂交（ＳＳＯＰＨ）方法，对中国汉族１２００例正常人ＨＬＡ－Ｂ２７基因进行分析。结果表明，在中国汉族中至少存在４种亚型，其中Ｂ２７０４亚型频率最高，约占Ｂ２７阳性正常人的５０．８５％，其次Ｂ２７０５，约占４０．６８％，其余为Ｂ２７０４／０５、Ｂ２７０６和Ｂ２７０３，分别占３．３９％、３．３９％和１．６９％。与国外有关资料相比，显示不同人群ＨＬＡ－Ｂ２７亚型分布频率差异较大。这一结论对研究ＨＬＡＢ２７与强直性脊柱炎的相关性有一定的作用。

HLA-B27 represents a family of closely related antigens. Six alleles differing in limited number of nucleotide substitutions have been described(B2701─B2706).So far only Caucasian and Gamgians (Africa)had been investigated.We have subtyped HLA─B27 in HLA─B27 positive healthy controls in Chinese Han nationality for the first time. Polymerase chain reaction was designed to amplify regions of class 1(exon 2 and 3).Amplified sequences were tested with eight sequence-specific oligonucleotides to distinguish all HLA-B27 subtypes,At least four B27 subtypes exist in our population. B2704 and B2705 were the most common subtypes showing presence of approximately 50.85%and 40.68% respectively.We believe that the above results will be helpful in the further research on the relationship between HLA-B27and ankylosing spondylitis.