白细胞介素－1和阿片肽促进大鼠大脑皮层神经细胞c－fos及c－jun　mRNA表达

INTERIEUKIN-1 AND OPIOID PEPTIDES INDUCE c-fos AND c-jun mRNA EXPRESSION OF RAT CORTICOCEREBRAL CELLS

对白细胞介素－１（ＩＬ－１）、脑啡肽、β－内啡肽及即刻早期基因ｃ－ｆｏｓ、ｃ－ｊｕｎ与癫痫发病机理的研究。结果显示ＩＬ－１、ＩＬ－１受体拮抗剂、β－内啡肽、抗β－内啡肽抗血清、亮－脑啡肽（ＬＥ）均能促进大脑皮层神经细胞ｃ－ｆｏｓ、ｃ－ｊｕｎｍＲＮＡ表达，但ＩＬ－１受体拮抗剂、抗β－内啡肽抗血清促ｃ－ｆｏｓ、ｃ－ｊｕｎｍＲＮＡ表达量明显低于ＩＬ－１、ＬＥ及β－内啡肽的作用，并能部分抑制后者的作用；ＩＬ－１、β－内啡肽、ＬＥ促ｃ－ｆｏｓｍＲＮＡ表达在一定范围呈现量效关系；ＩＬ－１诱导ｃ－ｆｏｓ、ｃ－ｊｕｎｍＲＮＡ表达呈现时间效应关系，均为短暂表达。由于具有不同生物学效应的因子均能诱导不同程度ｃ－ｆｏｓ、ｃ－ｊｕｎｍＲＮＡ表达，提示它们对靶基因的调控具有正性及负性双向性，对神经细胞兴奋性产生不同作用。

The interleukin-1(IL-1),enkephalin,β-endorphin and immediate early genes c-fos and c-jun are intimately related to the pathogenesis of epilepsies. Our results showed that IL-1,IL-1 receptor antagonist(IL-lra),β-endorphin,antiserum of anti-β-endorphin,leuenkephalin enhanced c-fos and c-jun mR-NA expression of rat cortico-cerebral cells. Induction of c-fos and c-jun mRNA by IL-lra and antiserum of anti-β-endorphin was less than that by IL-1,leu-enkephalin and β-endorphin. IL-lra and anti-serum of anti-β-endorphin partly inhibited the effect of IL-1 and β-endorphin ,respectively.The c-fos mRNA expression induced by IL-1,leu-enkephlin and β-endrophin revealed dose response curve at a scale of certain doses. The induction of c-fos and c-jun by IL-1 also revealed time course and was transient expression. Based on the different levels of c-fos and c-jun mRNA expression induced by various factors which have different biological effects,our results suggested that fos and jun acting as third messenger molecules and transcriptional factor,have biphasic regulation in target genes and different actions on the excitation of corticocerebral cells.