NIDDM患者不同血管并发症ApoE基因型频率分布的研究

THE DISTRIBUTION OF APOLIPOPROTEIN E GENOTYPE AMONG DIFFERENT VASCULAR COMPLICATIONS IN NON-INSULIN-DEPENDENT DIABETES MELLITUS

采用多聚酶链反应（ＰＣＲ），研究ＮＩＤＤＭ患者不同血管并发症ＡｐｏＥ基因型及其等位基因频率分布。结果：（１）并发冠心病（ＣＨＤ）组∈３／３基因型频率为５２％，显著低于对照组７６％（Ｐ＜０．０１）；∈４／３基因型频率为３４％，显著高于对照组１２％（Ｐ＜０．０１）；∈４等位基因频率为２１％，显著高于对照组９％（Ｐ＜０．０５），∈３频率为７４％，显著低于对照组８６％（Ｐ＜０．０５）。（２）大血管病变组∈３／３、∈４／３、∈４频率分别为５６％、３０％、２０％；∈３／３低于，∈４／３、∈４高于对照组（Ｐ＜０．０５）。（３）高血压组和微血管病变组亦有类似变化，但差异无统计学意义。因此推断，∈４等位基因增加ＮＩＤＤＭ患者血管并发症，尤其是ＣＨＤ的危险性。

Polymerase chain reaction was used in this study. The results showed:(1)the prevalence of genotype∈3/3 was about 52%in coronary atherosclerotic heart disease(CHD)group,which was significantly lower than 76%in control(P<0. 01);the prevalence of ∈4/3 was about 34%in CHD group,which was significantly higher than 12%in control(P<0.01);the prevalence of ∈4 allele was about 21%in CHD group, which was significantly higher than 9% in control(P<0.05);the prevalence of ∈3 allele was about 74%,which was significantly lower than 86%in control(P<0.05).(2)the prevalence of ∈3/3,∈4/3,∈4 in macrovascular complication group were about 56%,30%and 20%respectively,which were significantly lower(∈3/3) or higher(∈4/3,∈4)than those in control respectively(P<0.05).(3)there were similar apolipoprotein E genotype changes in both hypertension and microvascular complication groups,though the differences were not statistically significant. Therefore,we infer that ∈4 allele increases the risk for vascular complications,especially CHD in NIDDM.