叶绿酸对7，2—二甲基苯蒽诱发大鼠乳腺癌的影响

EFFECTS OF CHL OROPHYLLIN ON INCIDENCE OF MAMMA-RY TUMOR INDUCED BY 7,12-DIMETHYLBEN(a)AN-THRACENE IN RAT

本文研究了叶绿酸（ＣＨＬ）对７，１２—二甲基苯蒽（ＤＭＢＡ）诱发大鼠乳腺癌的影响。４３天龄雌性Ｓｐｒａｇｕｅ—Ｄａｗｌｅｙ大鼠随机分为３组，每组２５只。给ＤＭＢＡ前Ⅰ组给自来水，Ⅱ、Ⅲ组在饮水中加ＣＨＬ１．５ｍｍｏｌ，ｌｗｋ后每只大鼠一次灌胃给ＤＭＢＡ１０ｍｇ．给ＤＭＢＡ后Ⅰ、Ⅱ组给自来水，Ⅲ组饮水中继续加ＣＨＬ，持续２４ｗｋ。结果３组大鼠乳癌发生率分别为５０．０％、３３．３％和２３．８％．给ＣＨＬ的两组与对照组比较有下降趋势，但差异无显著性，说明ＣＨＬ的抗癌作用还有待进一步证实。实验中未发现ＣＨＬ有促癌作用。

In present study the effects of chlorophyllin,an antimutagen,on incidence of mam-mary tumor induced by 7,12 dimethylben(a)anthracene(DMBA)in rat were investigated.43 days old female Sprague Dawley rats (weighted around l00g)were randomly divided in-to 3 groups with 25 in each. A week before the the administration of DMBA,rats in group Ⅱand group Ⅲ were given sodium, copper chlorophyllin(CHL) 1.5 m mol/L in drinking water.Rats in groupⅠwere given tap water as control. On 50th days each animal was given DMBA10mg/Kg dissolved in corn oil by gavage. Rats in groupⅠand groupⅡwere given tap waterthereafter, while rats in group Ⅲ were given CHL throughout the experiment.The resultsshowed that the tumor rates in these groups were 50.0%,33.3%and 23.8% respectively。There were decressed of the rates in the two CHL treated groups though the differences a-mong the three groups were not significant. These data demonstrated that the anticarcino-genic effect of CHL need to be studied further. No tumor promoting effect of CHL was ob-served in above mentioned animal model.