甘蓝汁的抗诱变作用及其机理研究

STUDIES ON THE ANTIMUTAGENIC EFFECT AND ITS MECHA-NISM OFJUICE FOR B. OLERACEA

本文通过微核试验和肝组织中谷胱甘肽－Ｓ－转移酶（ＧＳＴ）活性、还原型谷胱甘肽（ＧＳＨ）含量及细胞色素Ｐ４５０含量测定，在哺乳动物整体水平，从遗传学和生化毒理学角度研究了甘蓝汁的抗诱变作用及其机理。结果发现，甘蓝汁对环磷酰胺（ＣＰ）诱发的小鼠骨髓多染红细胞（ＰＣＥ）微核细胞率有显著抑制作用，抑制率达４７．２％。Ｗｉｓｔａｒ大鼠饮甘蓝汁１０ｄ后，肝脏ＧＳＴ活性增加２５．０％，ＧＳＨ含量提高４７．８％，细胞色素Ｐ４５０含量增加３８．３％。

In the precent study the dismutagen effect of Cabbage juice (Brassica oleraceaVar. Capitata)on cyclophosphamide(CP)induced micronuclear on PCEs in mice bone mar-row and the effect on glutathione-s-transferase (GST)activity, glutathione (GSH) leveland cytochrone P450 content in the liver of rats were investigated. The result showed that theCP induced micronucleus rate was inhibited by 47.2%. The GST activity, GSH level andP450 content were increased by 25.0%,48.0%and 37.3%,respectively,after drank the juicefor 10 days.