叶绿酸铜钠的抗诱变作用及其机理

ANTIMUTAGENIC POTENTIALS AND MECHANISMS OFCHLOROPHYLLIN ON EFFECTS OF CYCLOPHOSPHAMIDE

本研究运用小鼠骨髓细胞微核心试验评价叶绿素的衍生物——叶绿酸铜纳（ＣｈｌｏｒｏｐｈｙｌｌｉｎＳｏｄｉｕｍ—ＣｏｐｐｅｒＳａｌｔ，ＣＨＬ）抑制间接诱变剂环磷酰胺（Ｃｙｃｌｏｐｈｏｓｐｈａｍｉｄｅ，ＣＰ）的诱变作用。实验根据小鼠给予ＣＨＬ和ＣＰ的时间，设计４种不同程序，以此探索ＣＨＬ抗诱变作用的机理．实验结果表明，ＣＨＬ本身无诱变作用，但对ＣＰ诱导微核作用有明显的抑制。同时也提示ＣＨＬ对ＣＰ的抗诱变作用可能至少有２种机理。Ｔｈｅｉｎｖｉｖｏｍｏｕｓｅｂｏｎｅ－ｍａｒｒｏｗＰＣＥｍｉｃｒｏｎｕｃｌｅｕｓｔｏｃｈｅｃｋｔｈｅａｎｔｉｍｕｔａｇｅｎｉｃｐｏｔｅｎｔｉａｌｓｏｆｃｈｌｏｒｏｐｈｙｌｌｉｎ（ＣＨＬ）ｏｎｍｕｔａｔｉｏｎｉｎｄｕｃｅｄｂｙｃｙｃｌｏｐｈｏｓｐｈａｍｉｄｅ（ＣＰ）ｗａｓｃａｒ－ｒｉｅｄｏｕｔ．ＩｎｏｒｄｅｒｔｏｅｘｐｌｏｓｅｔｈｅｐｏｓｓｉｂｌｅｍｅｃｈａｎｉｓｍｏｆａｎｔｉｍｕｔａｇｅｎｉｃｅｆｆｅｃｔｏｆＣＨＬ．Ｔｈｅｄｉｆ－ｆｅｒｅｎｔｔｒｅａｔｍｅｎｔｓｃｈｅｄｕｌｅｓｉｎｔｈｅａｄｍｉｎｉｓｔｒａｔｉｏｎｏｆＣＨＬａｎｄｏｆＣＰｔｏｍｉｃｅｗｅｒｅｄｅｓｉｇｎｅｄ．Ｔｈｅｒｅｓｕｌｔ?

The in vivo mouse bone-marrow PCE micronucleus to check the antimutagenicpotentials of chlorophyllin(CHL)on mutation induced by cyclophosphamide(CP)was car-ried out.In order to explose the possible mechanism of antimutagenic effect of CHL. The dif-ferent treatment schedules in the administration of CHL and of CP to mice were designed.The results indicated that CHL itself is not a mutagen,but showed significant inhibitory ef-fect against CP in inducing micronuclei. There should at least two mechanisms involved in theantimutagenic effect of CHL to CP.