小鼠着床后全胚胎培养方法的建立和应用

THE ESTABLISHMENT AND APPLICATION OF CULTURE OF POST-IMPLANATION WHOLE EMBRYO OF KUN-MINGMOUSE

本文报导昆明种小鼠着床后全胚胎培养方法。

In this paper,discribed the procedures for the culure of post-implantation embryoof Kun-Ming mouse. Half of the mouse embryos in uterue were explanted at early somitesstage (8.5-days) and grown in vitro by culture method described in this paper. Comparedwith littermates in vivo. very similar rate of growth and morphodifferentiation were observedover a period of 48 hours. Mitomycin C(MMC) and N,N-methylene- bis (2-amino-1,3,4-thiadiazole)(NSC-143019) were added respectively to 48 hours culture of 8.5 days wholemouse embryos . Significant yolk sac and embryonic growth retardation, morphological abnor-malities of embryos,including unfused neural tube,deficits in axial rotation and tail ,heart de-fects were found in MMC and NSC-143019 treated groups。It was showedthat both MMCand NSC-143019 exerted teratogenic effects on embryo of mouse in vitro.