体内一体外试验检测不同组织中化学致癌物诱导的程序外DNA合成

DETECTION OF UNSCHEDULED DNA SYNTHESIS INDUCED BYCHEMICAL CARCINOGENS IN DIFFERENT TISSUES WITH INVIVO&INVITROTESTS

本实验采用高压氧下组织体外标记程序外ＤＮＡ合成（ｕｎｓｃｈｅｄｕｌｅｄＤＮＡｓｙｎｔｈｅｓｉｓ，简称ＵＤＳ）放射自显影检测法，体内给药后取组织于体外标记。对４ＮＱＯ、ＡＦＢ１、Ｂｅｎｚ、ＭＢＮ、ＤＭＢＡ、ＤＥＮ在Ｗｉｓｔａｒ大鼠不同组织中诱导的ＵＤＳ进行检测的结果表明：此６种化合物均呈阳性，ＵＤＳ出现部位比肿瘤靶器官广泛，靶器官的ＵＤＳ强度不一定比非靶器官大，ＵＯＳ的出现有相对的器官和细胞特异性，明显的ＵＤＳ常见于肺、肾、肝、肾上腺、胰腺和颌下腺等组织。本研究使人们对ＵＤＳ发生部位与肿瘤靶器官的关系有了更多的认识，不支持某些学者认为的ＵＤＳ部位仅见于肿瘤靶器官的观点。

Using autoradiograph for detection of Unscheduled DNA synthesis(UDS)in-duced in different tissues of Wistar rats、was examined after administration in vivo of 4NQO,AFB 1,Benz,MBN·DMBA and DEN. The rcsult suggested that all thesechemicals are posi-tiveinducers of UDS.The sites of UDS were more extensive than that of tumor target or- gans and the intensity of UDS in target organs did not higher certainly than non- target or-gans.There was relative organ and cell specificity in sites where UDS induced. The signifi-cant UDS was often encountered in lung,kindey,liver, adrenal gland,pancreas and submaxil-lary gland, as well as in some other tissues. This study provides more knowledge a bout therelationship between U DS sites and target organs of tumorigenesis and did not support theviewpoint that UDS sites could belooked upon exclusively tumor target organs。