应用微核测试法研究人胚细胞提取物抗γ—线效应

INHIBITORY EFFECTS OF HUMAN FETAL CELL EXTRACTION ON MICRONUCLEUS INDUCED BY γ RAYS IRRADIATION

本文应用小鼠骨髓细胞微核（体内）和人淋巴细胞体外核异常测试法，以γ—线为诱变因子，研究了人胚细胞提取物（胚提物）的遗传毒性和抗突变效应。结果表明，胚提物的体内外微核试验均无明显的遗传毒作用，并且在本实验剂模范围内（体内：１—５ｍｇ／ｋｇ体重胚提物，体外：０．３—３０ｕｇ／ｍｌ胚提物）表现出明显的拮抗γ—线（３—４Ｇｙ）诱发小鼠骨骼多染红细胞微核和人淋巴细胞微核的效应。结合文献讨论，作者认为胚提物来自人体，易于吸收，可望成为一种新的抗辐射和肿瘤化学预防的制剂。

The mutagenic and antimutagenic effects of human fetal cell extract(HFCE)werestudied with micronucleus test in bone marrow cells of mice(in vivo)and in the lymphocytesof human blood(in vitro).No statistical significant difference between the control group andgroups treated with HFCE was found.The micronucleus induced by γ rays irradiation weredecreased remarkably,while HFCE administered at the doses of 1 5mg/kg bw in vivo andO.3 30ug/ml in vitro。The results suggested that HFCE might be a new preparation for the anti radiation and chemical preventions of human tumors。