氧代赖氨酸抑制CHL细胞有丝分裂作用和遗传毒性研究

INFLUENCE0F0XALYSINE ON THE MITOSIS OF CHL CELLSAND ITS GENOTOXIC STUDY

氧代赖氨酸是一种有抗癌作用的抗菌素，结构相似于赖氨酸。对ＣＨＬ细胞有明显的抑制有丝分裂作用，但并不诱发染色体畸变。用闪烁计数法定量测定肝细胞核的程序外ＤＮＡ的合成，未见氧代赖氨酸对其有明显影响。氧代赖氨酸与小牛胸腺ＤＮＡ分子相互作用的体外试验亦未见ＤＮＡ的ＣＤ谱明显改变。但氧代赖氨酸却明显地抑制肝细胞蛋白质合成。可以假设，氧代赖氨酸抑制了蛋白质合成，特别是组氨酸，因而抑制细胞分裂，并非对ＤＮＡ直接作用之故。未检测出氧代赖氨酸的遗传毒性。

Oxalysine was found to be an antitumor antiblotic with a simple chemical struc-ture very similary to lysine。In CHL cells it showed potent inhibition of mitosis,but had noeffect on chromosome aberration。The influence of oxalysine on DNA-repair was detectedafter incubation of cultured rat hepatocyles with oxalysine and[3H]-TdR in the presence ofhydroxyurea,using nuclear isolation-liquid scintillation counting.Oxalysine did not induceUDS in hepatocyles。Further studies on effect of oxalysine on DNA conformation indicate thecircular dichroism spectra of calf thymus DNA were not altered by oxalysine at 50μ g/ml。It isthought that inhibition of mitosis caused by oxalysine results not from effect on DNA, butmore likely,from inhibition of protein biosynthesis,in particular the synthesis of histone。 Oxalysine did not show any genotoxicity。