肺癌癌基因蛋白产物同步检测的对比分析

COMPARATIVE STUDY OF CO-EXPRESSION OF ONCOCENE PROTEIN PRODUCTS IN LUNG CANCER

用免疫组织化学方法对７４例肺癌连续切片组织同步进行了ｒａｓ、Ｃ－ｍｙｃ、Ｐ５３三种癌基因蛋白产物的检测。结果显示ｒａｓＰ２１、Ｃ－ｍｙｃＰ６２、和Ｐ５３阳性表达率分别为７１．６％、６４．８％和３３．８％。而癌旁支气管粘膜及腺体分别为１６．１％、２２．６％和０。Ｐ２１和Ｐ６２之间的阳性率无统计学差别（Ｐ＞０．０５），表明这二个癌基因在肺癌发生发展中具有同等的重要性。有两种以上癌基因蛋白同时表达阳性者有４６例（６２．２％），１０例（２１．７％）同时有Ｐ２１、Ｐ６２和Ｐ５３共同表达，结果提示肺癌在发生发展过程中多需要两种或两种以上癌基因作用的结果。

Co-exprssion of protein produces of omcogene ras ,c-myc,and P53were synchronously detected and compared by immunohistochemical method in serial sec-tions of 74 lung cancers including 31 cases of surrounding bronchial epithelium and glands.The rexults showed that the positive stainhg of ras, myc,and P53 were found in 71.6%,64.8% and 33.8% of lung cancer,whereas only 16.1%,22.6%and 0%intissues surroundingthe cancer.No significant difference Was found between1 ras P21 and myc P62 expressionin lung cancer(P>0.05).The results indicated that both ras and myc oncogenes have thesame importan in the carcinogenesis and progression of lung cancer The detection forthree oncogene expression in each case showed that there were 62.2 % cases in lung can-cer with co-expression and 6.5% n cancer-surrounding brounding bronchial proliferative epithelium.Co-expression of ras+myc,ras+P53, myc+P53 and ras+myc +P53 in lung cancer was52.2%, 15.2%,10,9%,and 21.7%,respectively The results suggested that multioncogenechanges of ras myc and P53 were involved in occurrance of lung cancer.