维吾尔族宫颈癌中H－ras癌基因第12位密码子突变的研究

DETECTION OF H-RAS GENE MUTATIONS AT CODON 12 IN UYGURIAN CERVICAL CARCINOMA

维吾尔族宫颈癌组织中Ｈ－ｒａｓ癌基因３１２ｂｐ含第１２位密码于的ＤＮＡ顺序经聚合酶链反应体外扩增，结合限制性内切酶ＭｓｐＩ限制片段长短的多态现象分析，以检测第１２位密码子突变，结果在５３例官颈癌中只检出１例存在第１２位密码子突变，在２０例慢性宫颈炎或宫颈糜烂中无１例检出。说明Ｈ－ｒａｓ癌基因第１２位密码子突变可能不是维吾尔族官颈癌中癌基因激活的主要方式。

The 312bp DNA sequence con atining codon 12 of H-ras oncogenein Uygurian cervical carcinoma was amplified by the polymerase chain reaction(PCR),andthen the restriction fragment length polymorphisms of the amplified sequence was analy-zed using the restriction endonuclease Msp I to detect mutations at codon 12.Point mu-tations at codon 12 were found in 1 of 53 Uygurian cervical carcinomas. There were nopoint mutations at codon 12 in any of 20 patients with chronic cervicitis and cervicalerosions. These observations sugges that H-ras gene mutations at codon 12 may not bethe main oncogene activation mechanism in Uygurian cervical carcinoma.