肺癌及肺鳞癌癌前病变中K-ras基因点突变的研究

A STUDY ON THE POINT MUTATION OF K-RAS ONCOGENE IN HUMAN LUNG CANCER AND PRECANCEROUS LESIONS OF LUNG SQUAMOUS CELL CARCINOMA

应用ＰＣＲ技术及限制性片段长度多态性分析方法，研究了４１例非小细胞肺癌和１４例肺鳞癌癌前病变中的Ｋ—ｒａｓ基因第１２位密码子点突变。结果在非小细胞肺癌中检出 １０例Ｋ—ｒａｓ基因点突变，突变率为２４．４％；而在１４例肺鳞癌癌前病变中未检出一例，这提示Ｋ—ｒａｓ基因点突变虽然不发生在肺鳞癌癌前病变阶段，但仍与非小细胞肺癌的发生有密切关系。

The point mutation in codon 12 of k-ras oncogene in 41 human lung cancer and 14 precancerous lesions of lung squamous cell carcinoma was studied by Polymerase Chain Reaction(PCR) and Restriction Fragment Length Polymorphisms (RFLP). The Point mutation of k-ras oncogene was found in 10 of 41 non-small cell lung cancer(24.4%), but was not found in 14 precancerous lesions of lung squamous cell carcinoma. Tne results showed that k-ras mutation did not exist in precancerous lesions of lung squamous cell carcinoma, but associated with non-small cell lung can- cers.