摘要
应用检测肿瘤ＤＮＡ多态性的探针，对１５例大肠癌进行了ＬＯＨ分析。结果，探针ｐ１０－５（１７ｐ１３．１）在检出的８个信息个体中有５例发生了ＬＯＨ（占６２．５％）。而ｐＹＮＺ２２．１（１７ｐ１３．３），ｐ５３，ｐＭＳ１－３７（３ｐ１４．２）和ＤＲ７８（１ｐ２１－ｑｔｅｒ）探针的同源区并未检出ＬＯＨ。对１０例大肠癌还进行了ｐ５３ｍＲＮＡ的Ｎｏｒｔｈｅｒｎ印迹分析，发现全部被检测样品均有一条２．８ｋｂ的ｐ５３ｍＲＮＡ杂交带。１０例中６例ｐ５３ｍＲＮＡ表达水平高于配对的正常组织，３例则降低。本研究提示，ｐ５３基因突变存在明显的异质性，同时还说明１７ｐ的ＬＯＨ可能是大肠癌发生过程中的一个至关重要的分子事件。
We have analyzed the LOH(loss of
heterozygosity) in tumorDNA from 15 patients with colorectal cancer
using probes that detecting TFLP. LOHaffecting p10-5( 17p13.1) was
observed in 5 of 8 (62.5%)of heterozygous patients,while LOH of
pYNZ22.1(17p13.3),p53, pMS1─37(3p14.2),and DR78(1p21─qter) locus
was not observed. Northern analysis of mRNA expression of p53 gene
wasperformed in 10 patients. Each of the samples examined showed
expression of a single2.8─kilobase RNA species. Overexpression of
p53 mRNA in tumors was observed in6 of 10 patients,but expression at
low level was observed in 3. These data suggest thatmutations in p53
gene is heterogeneous and that LOH on chromosome 17p may be acritical
molecular evemt during colorectal─tumor development.
出处
《癌症》
SCIE
CAS
CSCD
北大核心
1995年第5期331-333,共3页
Chinese Journal of Cancer
