茶多酚对体外人肝癌细胞的生物效应

EFFECT OF CHINESE GREEN TEA POLYPHENOLS(GTP)ON HUMAN LIVER CANCER CELL IN VITRO

作者以细胞数、细胞分裂指数、标记指数，标记有丝分裂指数等为观察指标，研究了茶多酚（ＧＴＰ）对体外培养的人体肿癌细胞ＳＭＭＣ—７７２１的抗肿瘤活性，并与正常人肝细胞作比较。结果：０．０２５ｍｇ／ｍｌ和０．０５ｍｇ／ｍｌ的ＧＴＰ对肝癌细胞的生长有明显的抑制作用，细胞分裂指数明显下降，能阻滞细胞周期的各个进程，并与剂量有关，较大剂量的作用较明显；ＧＴＰ对正常人肝细胞无上述作用。本实验为茶多酚开发应用提供了理论依据。

Anticancer action of chinese green tea polyphenols(GTP) on human liver cancer cell was studied in vitro. Tne proliferation numbers of cells in culture ,mitotic index (MI), labeling index(LI)and propostion of labeled mitosis(PLM) were observed and compared with human normal liver cells. The results showed that(1) GTP at 0. 025 and 0. 05mg/ ml could sighificantly innibit the growtn of liver cancer cell;( 2) MI of the two groups of GTP were significantly lower tnan that of control;( 3) GTP could block the every migration of liver cancer cell from Gl to M phase. These effects were related witn the dosage of GTP. Inhibitory effect at 0. 05mg/ml was stronger than that at 0.025mg/ml,The result also showed that GTP hadn′t above effect on human liver cells. The study would provide theoritical basis for using GTP.