荧光法测定羽毛中的色氨酸含量

Measurement of the Content of Tryptophan in Feather With Fluorescence Method

本文探讨了羽毛中色氨酸的荧光分析方法，将２～５ｍｇ的羽毛样品置于聚四氟乙烯管中，用１ｍｌ含０．５％可溶性淀粉的５ｍｏｌ／Ｌ ＮａＯＨ为水解液，以纯氮为保护气体，在１１０℃水解２０小时，水解产物用６ｍｏｌ／ＬＨＣｌ中和，然后用Ｎａ２ＨＰＯ４－ＮａＯＨ的缓冲溶液（ｐＨ１１）稀释５倍，在激发波长２８０ｎｍ，发射波长３６２ｎｍ测定荧光强度及其浓度。经多次试验，结果较为满意，重现性较好，其相对标准偏差为０．６１％～３．００％。样品的回收率为９５．３％～１０２．１％。此法快速、简便，适用于各种羽毛的例行分析。

This paper probes into the fluorescene analysis method of tryptophan in feathers.2～5mg feather sample was placed in the polytetrafluoroethylene tube.1 ml of 5mol/L NaOH comtaining 0.5% soluble starch was used as hydrolysate with pure nitrogen as protective gas.Hydrolyzate was hydrolyzed at 110℃ for 20 hours and neutralized with 6mol/L HCl.Then it was diluted five times with the buffer Na2HPO4-NaOH (pH11).The intensity and concentration of the fluorescence were 280nm of stimulated wavelength and 362nm of fluorescene wavelength.The results were reproducible.Compared with the standard,its deviation was 0.67%～3.01%.The recovery rate of samples was 95.3%～102.1%.