摘要
本文分别用EcoRI、HindⅢ、BamHⅠ、HaeⅢ限制性内切酶对U937细胞基因组DNA和正常人基因组DNA进行Southernblot分析,结果表明U937细胞存在C──fos基因限制性多态性改变,并伴随着基因扩增。
The genomic DNA from U937 cells and normal
human PMNCs was analysed throughSouthern blot with the restriction endonuclease EcoRⅠ,Hind
Ⅲ,BamH Ⅰ,Hae Ⅲ.The results showedthat there was a novel band in U937 cells compared with
the normal human genome,and strength of theband was bigger than that of normal genome. These
data indicate that there are structural changes andamplification of c──fos gene in U937 cell line.
出处
《白求恩医科大学学报》
CSCD
1995年第2期120-122,共3页
Journal of Norman Bethune University of Medical Science