正常成人和肿瘤患者血清纤维凝集素含量的放免分析

Detection of fibronectin in serum of normal human and patients with carcinoma

纤维凝集素与许多疾病、特别是与肿瘤的发生发展有着密切的关系。目前国内尚未见对正常国人及肿瘤患者血清纤维凝集素水平测定的报道。本研究从正常国人血浆中提纯纤维凝集素，以此制备了抗体，并建立了放射免疫测定方法。用此方法测定了３ｌ０名正常成人血清纤维凝集素的水平（０．４１±０．１９ｍｇ／ｍＬ）和ｌ０９例肿瘤术前与治疗前纤维凝集素的水平（０．０４±０．０４ｍｇ／ｍＬ）。其结果二者有显著差异（Ｐ＜０．０１）。

There is a close relation between fibronectin(FN)and many diseases,especially carci-noma. At present,there is no report on the detection of level of FN in serum of normal human and pa-tients with carcinoma. In this paper, FN was purified from plasma of mormal human. Then,we pre-pared antibody of antifibronectin whth our own FN and established radioimmunoassay. We used themethod to meassure FN level in (310)normal human (0.4±0. 19mg/mL)and in ( 109)patientswith carcinoma (0. 04±0.041mg/mL)who were never operated or treated. The ressults showed re-marrkable difference.(P<0. 0l ).