摘要
本文将人转化生长因子β１（ｈＴＣＦβ１）全长ｃＤＮＡ克隆至真核表达载体ｐＭＡＭｎｅｏ的ＮｈｅⅠ位点，利用ＤＥＡＥ一Ｄｅｘｔｒａｎ法将ｈＴＧＦβ１载体导入ｃｏｓ一７细胞中。实验结果表明：所构建的表达载体在ｃｏｓ一７细胞内有转录水平的表达，这为我们进一步研究ｈＴＧＦβ１在其它细胞内的表达与调控之间的关系奠定了基础。
The full length cDNA of
transforming growth factorβ1(TGFβ1)was cloned into theNhe l site of mammalian expression
vector pMAMneo and transfected in cos一7cells ty means ofDEAE一Dextran. The results showed
that the eukaryotic gene TGFβ1 had transcriptional expressionin cos一7cells. The construction of
TGFβ1 expression vector laids foundation for studying the relation- ship between the expression
and regulation in tumor cell.
出处
《白求恩医科大学学报》
CSCD
1995年第3期235-237,共3页
Journal of Norman Bethune University of Medical Science
基金
国家自然科学基金
