抗CRF单克隆抗体制备及鉴定

Preparation of anti-CRF monocl onal antibody

本文报道了应用促肾上腺皮质激素释放因子（ＣＲＦ）在碳化二亚胺作用下与牛血清白蛋白（ＢＳＡ）偶联形成的完全抗原，免疫Ｂａｌｂ／ｃ小鼠，采用淋巴细胞杂交瘤技术。制备了２株分泌抗ＣＲＦ单克隆抗体的杂交瘤。并利用建立的小分子肽ＣＲＦ的间接ＥＬＩＳＡ法，对其腹水效价及其特异性进行了分析。

Using hybridoma technique, 2 hybridoma cell lines secreting monoclonal antibody(McAb)against CRF were obtained.The titer pf McAb were assayed by ELISA。 The crossreactivitiesof McAb with NPY,ACTH,FSH and LH were not visible in the system。