76例宫颈癌组织中人类乳头瘤病毒的检测

Analysis of HPV in 76 cases of cervical cancer

本文应用ＨＰＶ－ＰＣＲ技术和ＤＮＡ杂交技术对７６例宫颈鳞癌标本进行了ＨＰＶ－ＤＮＡ的研究，发现ＨＰＶ－ＤＮＡ存在率为９０．７９％（６９／７６），主要类型为ＨＰＶ１６和１８。多酶切ＳｏｕｔｈｅｒｎＢｌｏｔ杂交证实在宫颈癌中９２．５６％的ＨＰＶ－ＤＮＡ发生变异，说明ＨＰＶ－ＤＮＡ的基因变异与其致癌作用密切相关。ＰＣＲ结果证实ＨＰＶ－Ｅ６片段在宫颈癌组织中常可出现，推测可能是致癌的关键片段之一。因此，检测宫颈癌组织中ＨＰＶ－ＤＮＡ的完整性、关键基因片段的存在与否。

In this paper, HPV-PCR and DNA hybridization technique were used to study 76cases of cervical squamous cancer. HPV-DNA was found in 69 cases(90.79%). The main typieswere HPV16 and l8. The commonest type was HPV16. By Southern Blot,HPV-DNA was identifiedin 54 cases (92.56%)of cervical cancer.Ihe results suggested that HPV was closely related to onco-genicity and that HP-E6 fragment was one of key fragments to induce cervical cancer. So the examina-tion of intactness and key fragment of HPV-DNA was significant to predict the prognosis of HPVinfection.