摘要
研究分析超低温冻存前后外周血淋巴细胞（ＬＣ）的转化活性改变。结果发现，冻存后Ｔ淋巴细胞（ＴＬｃ）对植物凝集素（ＰＨＡ）刺激反应迟缓，转化率减低（冻前７２小时为６６．１９％，冻后７２小时为４８．９３％），延长培养时间（９６小时）可使部分细胞功能得以恢复，但仍不能达到冻前水平（冻前６５．６９％，冻后５８．２％）。冻存后ＴＬＣ转化中的母细胞型和分裂型百分比率下降明显，前者由３８．３２％减至２３．４８％，后者由１．２９％减１０．３６％。说明超低温冻存可影响ＴＬＣ内蛋白质和核酸合成。对Ｂ淋巴细胞（ＢＬＣ）影响不明显。
The transformation activities
of pre- and post-cryopreservatory peripberal lymphocytes wereanalysed.It was found that
post-cryopreservation,the reactivity of TLC to PHA was delayedand their transformation rate
was reduced(66.19%of unfrozen cells vs 48.93% of frozen cell)。Enlongation of the duration of
cultivation(96h)can restore the function of a part of frozen cells(at 96th h, 65.69%of unfrozen
cells vs 58.2%of frozen ones).With the assay of TLCtransformation,it was found that the
portions of blastic type and mitotic type decreasedsignificantly(from 38.23%to 23.48%for the
former,from 1.29%to 0.36%for the latter,respectively).It、was suggested that with
cryopreservation,the process of transformation wasinhibited by the disturbance of the
synthesis of protein and nucleic acid on TLC. BLC had noobvious change.
出处
《白血病》
1995年第2期91-93,共3页
