摘要
建立了用染色体特异的探针检测细胞染色体畸变的原位杂交法。用生物素标记的PY3.4探针对12例男性胃癌标本进行原位杂交,检测Y染色体的丢失,我们发现:胃癌组织细胞的Y染色体丢失率很高,Y染色体丢失很可能是胃癌细胞的一个染色体标志,胃癌组织细胞的Y染色体丢失与肿瘤的分化程度和肿瘤的转移有一定关系,这对胃癌的诊断和估计预后有一定帮助。
his report investingated the use of in situ
hybridization for detection of chromtosome aberrations with chro-
mosome-specific DNA probes. Fluorescent in situ hybridization(FISH)
with biotinylated PY3.4 probe was used for the detection of loss of
the Y chromosome of twelve male gastric carcinomas,.We found that the
loss of Y chromosome from the gastric carcinoma was very significant,
it possibly represented a chromosomal marker in this type of
cancer and would be useful to diagnosis of gastric carcinoma. We also
found that the loss of Y chromosome from the gastric carcinoma
correlated with the degree of differentiation and the metastasis of
tumors,which would help estimate prognosis of tumor.
出处
《北京医科大学学报》
CSCD
1995年第2期87-89,共3页
Journal of Peking University(Health Sciences)
基金
国家"八五"攻关课题。
关键词
胃肿瘤/遗传学
染色体缺失
原位杂交
Headings Stomach neoplasms/genetics
Chromosome delection
Situ
hybridization