PCR和SSO探针分析HLA－DQ位点的基因型探讨

A STUDY OF HLA-DQ LOCI GENOTYPING BY PCR-SSO

用ＰＣＲ－ＳＳＯ方法对４２名汉族常人ＨＬＡ－ＤＱＡ、ＤＱＢ位点多态性等位基因进行了基因型分析。结果发现，ＤＱＡ位点有２０种基因型，ＤＱＡ０１０１／０１０３占２１％，ＤＱＡ０１０１／０５０１占１１．９％。ＤＱＡ０１０１／０１０３、０１０１／０５０１和０１０１／０６０１三种杂合子基因型占研究人数的４２．８％。ＤＱＢ位点２１种基因型，以ＤＱＢ０６０１／０６０４ｂ为最高，占１９％，ＤＱＢ０６０１／０４０２和ＤＱＢ０６０１／０３０１各占１１．９％。只一例ＤＱＢ０６０１／０６０１为纯合子基因型。

HLA-DQA and DQB genotyping for 42 normal individuals of Han Nationality by ploymerase chain reaction-se-quence-specific oligonucleotides probe hybridization was described.The results showed 20 DQA and 21 DQB geno-types respectively.DQA0101/0103 heterozygous gene frequency was as high as 21%,DQA0101/0501 11.9%.DQB 0601/0604b heterozygous gene frequency in DQB locus was the highest,19%.It was discovered that DQB0601/0601 homozygous genotype was the only one.