应用几种青霉素基本结构提高体外诊断青霉素过敏能力──一个新试验系统

Using penicillin-based structures to elevate the diagnostic rate of penicillin allergy in vitro──a new test system

采用新的共价结合方法，将几种青霉素基本结构的β内酰胺和噻唑烷环分别结合至氨基臂纸片。以ＲＡＳＴ测定５３例有育霉素过敏史者血清中特异性ＩｇＥ浓度，并与国际通用商品ＰｈａｄｅｂａｓＲＡＳＴ系统相比较；结果显示：新系统阳性率由ＰｈａｄｅｂａｓＲＡＳＴ系统的３７．７％上升至５４．７％。新系统不仅提高了体外诊断能力，而且提供了选择使用安全的β内酰胺类杭茵药物的可靠依据。

A new covalent coupling method was used to make the β-lactan and thiazolidine ring of various penicillin-based structures attach to amino-spacer discs repectively.The allergen discs with above species were used in the radioallergosorbent test(RAST)to measure the concentrations of specific IgE in the sera of 53 subjects having a history of penicillin allergy and compared with the commercial Phadebas RAST system for evaluation.The results showed that the use of several penicillinbased structures in a new system increased the positive rate to 54.7%from 37.7%utilizing Phadebas RAST. Thus,the new test improves the capabilities for detecting allergy to penicillin in vitro and offera good possibility of selecting a safe β-lactan antibiotic for penicillin-allergic individuals.